Determination of Phenolic Compounds in Litchi Juices by Solid-Phase Extraction and High Performance Liquid Chromatography

Asian Journal of Chemistry 2013

Determination of Phenolic Compounds in Litchi Juices by Solid-Phase Extraction and High performance Liquid Chromatography

Min Wu，ZhuoYan Hu, Ping Zheng and Luan Zhang

摘要：A simple analytical method for the quantitative determination of phenolic compounds in litchi juice was developed. The phenolic compounds in litchi juice were extracted by solid-phase extraction using Bound Elut C18 cartridges and the extract was analyzed by HPLC coupled with UV detection. Solid-phase extraction conditions were as follows: samples were adjusted to pH 3.0, cartridges firstly were washed by 5 mL 0.01 M HCl then 5 mL distilled water, at last phenolic compounds on C18 cartridge were eluted by 7 mL methanol. Elution were blowed to 1 mL by pressure blowing concentrator. Phenolic compounds separation effect was good and extraction rate was high in this solid-phase extraction procedure. This paper gave a method to extract and determine fastly phenolic compounds present in Litchi juice.

DOI：https://doi.org/10.14233/ajchem.2013.15400